Multiplex Polymerase Chain Reaction Used for Bovine Embryo Sex Determination

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction

Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted semen is tedious and expensive. In this study, a rapid, cost effective and reliable method for determining the sex ratio was developed using a...

Streptococcus pneumoniae Type Determination by Multiplex Polymerase Chain Reaction

The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77 isolates from clinical specimens collected from children at Seoul National University Children's Hospital from 2006 to 2010. These strains were selected as they represented 26 different serotypes previ...

Sex Determination by Polymerase Chain Reaction and Karyotyping of Bovine Embryos at First Cleavage in vitro

The purpose of this study was to compare the embryo sexing methods between PCR and karyotyping of in vitro produced (IVP) bovine embryos at the 2-cell stage. The embryos were divided into 3 groups according to the time of first cleavage completion. Zygotes cleaving before 24 hours postinsemination (hpi), during 24-36 hpi and after 36 hpi were classified as fast, intermediate and slow cleaving e...

Rfbo157 by Multiplex Polymerase Chain Reaction

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.